Recommanded Product: Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrateIn 2020 ,《High Protein-Loading Silica Template for Heterogeneous Protein Crystallization》 appeared in Crystal Growth & Design. The author of the article were Chen, Wenqian; Park, Sung Joon; Kong, Fanlu; Li, Xiaoyu; Yang, Huaiyu; Heng, Jerry Y. Y.. The article conveys some information:
As a purification technol., crystallization is advantageous over chromatog. and precipitation in terms of purity, cost and scalability. In general, proteins have slow crystallization kinetics due to their complex configurations, but this problem can be overcome with heterogeneous nucleants. High protein-loading mesoporous silica is a promising nucleant due to its favorable interaction with protein. The current study employs such a template in the batch crystallization of lysozyme and thaumatin at 1 mL scale. Using lysozyme as the main model protein, the results show that the template had high protein loading (220 – 300 mg lysozyme/g silica) and induced significantly faster crystallization as compared to the unseeded samples over a wide range of initial protein concentration (10.0 – 47.5 mg/mL in the case of lysozyme) at 25° with 0.5 M potassium sodium tartrate tetrahydrate (precipitant) concentration It was found that the template had to be saturated with the target protein before the experiments to achieve faster kinetics or else it could delay the crystallization process. These findings were re-affirmed by the crystallization experiments of thaumatin. The current study demonstrates the effectiveness of high protein-loading silica as nucleant in protein crystallization and the importance of its pre-treatment with the target protein. In the experiment, the researchers used many compounds, for example, Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5Recommanded Product: Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate)
Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5) is a ferroelectric crystal with a high piezoelectric effect and electromechanical coupling coefficient. Recommanded Product: Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate Potassium sodium tartrate tetrahydrate has been used in the preparation of Lowry reagent for the determination of microsomal protein concentration in rat hepatic microsomes by Lowry method.
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