Xu, Bingbing; Wang, Zhiying; Wang, Ruijun; Song, Guoxin; Zhang, Yanjun; Su, Rui; Liu, Yongbin; Li, Jinquan; Zhang, Jiaxin published an article in 2022, the title of the article was Metabolomics analysis of buck semen cryopreserved with trehalose.Computed Properties of 473-81-4 And the article contains the following content:
Trehalose is commonly used as an impermeable cryoprotectant for cryopreservation of cells, but its cryoprotective mechanism has now not but been determined This study investigated the cryopreservation impact of trehalose on buck semen cryopreservation and finished metabolic profiling of freeze-thawed media by way of the GC-MS-based metabolomics for the first time. Metabolic pattern recognition and metabolite identification by means of principal component anal. (PCA), partial least squares discriminant anal. (PLS-DA) and metabolic pathway topol. anal. revealed the results of trehalose on buck sperm metabolism at some point of cryopreservation. The results confirmed that trehalose drastically progressed sperm motility parameters and structural integrity after thawing. PCA and PLS-DA anal. discovered that the metabolic patterns of the freezing-thawing media of buck semen cryopreserved with trehalose (T group) or without trehalose (G group, Control) were certainly separated Using screening conditions of VIP >1.5 and p vaule <0.05, a total of 48 differential metabolites have been recognized, whithin l-isoleucine, L-leucine, L-threonine, and dihydroxyacetone were notably enriched in valine, leucine and isoleucine biosynthesis, glycerolipid metabolism, and aminoacyl-tRNA biosynthesis pathways. In brief, trehalose can efficiently improve membrane structural integrity and motion parameters in buck sperm after thawing, and it exerts a cryoprotective impact with the aid of changing sperm amino acid synthesis and the glycerol metabolism pathway. The experimental process involved the reaction of 2,3-Dihydroxypropanoic acid(cas: 473-81-4).Computed Properties of 473-81-4
The Article related to trehalose buck semen cryopreservation metabolomic analysis, buck, cryopreservation, metabolomics, spermatozoa, trehalose, Biochemical Methods: Biological and other aspects.Computed Properties of 473-81-4
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