On February 28, 2022, Gautam, Veer Singh; Singh, Arti; Kumari, Puja; Nishad, Jay Hind; Kumar, Jitendra; Yadav, Monika; Bharti, Rajnish; Prajapati, Priyanka; Kharwar, Ravindra Nath published an article.Formula: C14H22O The title of the article was Phenolic and flavonoid contents and antioxidant activity of an endophytic fungus Nigrospora sphaerica (EHL2), inhabiting the medicinal plant Euphorbia hirta (dudhi) L.. And the article contained the following:
Since endophytic fungi are pivotal sources of various bioactive natural compounds, the present study is aimed to investigate the antioxidant compounds of the endophytic fungus Nigrospora sphaerica isolated from a pantropical weed, Euphorbia hirta L. The fungus was fermented in four different media and each filtered broth was sequentially extracted in various solvents. Crude extracts collected from different solvents were subjected to phytochem. anal. and antioxidant activity. The total phenolic content (TPC) and total flavonoid content (TFC) were maximal in Et acetate crude extract (EtOAcE) of endophyte fermented in potato dextrose broth (PDB) medium (77.74 ± 0.046mgGAE/g and 230.59 ± 2.0 mgRE/g) with the highest 96.80% antioxidant activity. However, TPC and TFC were absent in hexane extract of Czapek Dox broth (CDB) medium exhibiting the lowest 4.63 ± 2.75% activity. The EtOAcE (PDB) showed a pos. correlation between TFC and antiradical activity (R2 = 0.762; P < 0.05), whereas a high pos. correlation was noticed between TPC and antioxidant activity (R2 = 0.989; P < 0.05). Furthermore, to determine the antioxidant activity, EtOAcE (PDB) was subjected to TLC bioautog.-based partial purification, while GC/MS anal. of the partial purified extract was done to confirm the presence of phenolics along with antioxidant compounds that resulted in the detection of 2,4-Di-tert-butylphenol (13.83%), a phenolic compound accountable for the antioxidant potential. Conclusively, N. sphaerica is a potential candidate for natural antioxidant. The experimental process involved the reaction of 2,4-Di-tert-butylphenol(cas: 96-76-4).Formula: C14H22O
The Article related to nigrospora sphaerica euphorbia hirta phenolic flavonoid antioxidant, antioxidant activity, compounds purification, gc–ms analysis, n. sphaerica, phytochemical analysis, tlc bioautography and other aspects.Formula: C14H22O
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