Kandala, Divya T.; Del Piano, Alessia; Minati, Luca; Clamer, Massimiliano published the artcile< Targeting Translation Activity at the Ribosome Interface with UV-Active Small Molecules>, SDS of cas: 25055-82-7, the main research area is targeting translation ribosome interface UV small mol.
Puromycin is a well-known antibiotic that is used to study the mechanism of protein synthesis and to monitor ribosome activity due to its incorporation into nascent peptide chains. However, puromycin effects outside the ribosome catalytic core remain unexplored. Here, we developed two analogs (3PB and 3PC) of the 3′-end of tyrosylated-tRNA that can efficiently interact with several proteins associated with ribosomes. We biochem. characterized the binding of these analogs and globally mapped the direct small mol.-proteins interactions in living cells using clickable and photoreactive puromycin-like probes in combination with in-depth mass spectrometry. We identified a list of proteins targeted by the mols. during ribosome activity (e.g. GRP78) and we addressed possible uses of the probes to sense the activity of protein synthesis and to capture associated RNA. By coupling genome-wide RNA sequencing methods with these mols., the characterization of unexplored translational control mechanisms will be feasible.
ACS Omega published new progress about Animal gene, GRP78 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 25055-82-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H8N2O, SDS of cas: 25055-82-7.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts