COA of Formula: C13H19Br2ClN2OOn September 30, 2022 ,《Facile approaches for determination of Bromhexine Hydrochloride and its active metabolite Ambroxol Hydrochloride using Eosin Y》 was published in Annales Pharmaceutiques Francaises. The article was written by El-Sayed, A.; Elmansi, H.; Shalan, S.; Eid, M.. The article contains the following contents:
New validated Spectroscopic methods were developed to assay Bromhexine Hydrochloride and its active metabolite Ambroxol Hydrochloride sep. in pure form and pharmaceutical formulations. The spectrophotometric assay (method I) shows complex formation between each of the drugs and Eosin Y at 540 nm at pH 3.6 and 3.4 mL of 4 x 10-4M Eosin for Bromhexine and Ambroxol. The Spectrofluorimetric assay (method II) depends on quenching eosin native fluorescence by the studied drugs, which measured at 540 nm after excitation at 302 nm. The spectrophotometric absorbance-concentration plot is rectilinear over the ranges (1.0-5.0) and (1.0-10.0) μg/mL for bromhexine and ambroxol with LOD of 0.31 and 0.14μg/mL and LOQ of 0.94 and 0.42μg/mL for the two drugs resp. The fluorometric-concentration plot is linear along the range (1.0-5.0) μg/mL and (1-10) μg/mL for the two drugs resp. with LOD of 0.13μg/mL and 0.22μg/mL and LOQ of 0.4μg/mL and 0.65μg/mL for the two drugs, resp. Developed assays have been validated in agreement with ICH recommendations and they were used in the anal. of com. drug formulations containing the two mucolytic drugs and the results were matching with those obtained by the comparison method.De nouvelles methodes spectroscopiques validees ont etedeveloppes pour doser le chlorhydrate de bromhexine et son metabolite actif, le chlorhydrate d’Ambroxol, sous forme pure et dans des formulations pharmaceutiques. Le dosage spectrophotometrique (methode I) montre la formation d’un complexe entre chacun des medicaments et l’eosine Y a540 nm a pH 3,6 et 3,4 mL d’eosine 4 x 10-4M pour la bromhexine et l’ambroxol. Le dosage spectrofluorimetrique (methode II) depend de l’extinction de la fluorescence native de l’eosine par les medicaments etudies, qui a ete mesuree a 540 nm apres excitation a 302 nm. Le trace spectrophotometrique. Le trace spectrophotometrique absorbance-concentration est lineaire sur les plages (1,0-5,0) et (1,0-10,0) μg/mL pour la bromhexine et l’ambroxol avec LOD de 0,31 et 0,14μg/mL et LOQ de 0,94 et 0,42μg/mL pour les deux medicaments respectivement. Le trace de la concentration fluorometrique est lineaire le long de la plage (1,0-5,0) μg/mL et (1-10) μg/mL pour les deux medicaments respectivement avec LOD de 0,13μg/mL et 0,22μg/mL et LOQ de 0,4μg/ mL et 0,65μg/mL pour les deux medicaments, respectivement. Les tests developpes ont ete valides en accord avec les recommandations de l’ICH et ils ont ete utilizes dans l’analyze des formulations de medicaments commerciaux contenant les deux medicaments mucolytiques et les resultats correspondaient a ceux obtenus par la methode de comparaison. In the experimental materials used by the author, we found trans-4-((2-Amino-3,5-dibromobenzyl)amino)cyclohexanol hydrochloride(cas: 23828-92-4COA of Formula: C13H19Br2ClN2O)
trans-4-((2-Amino-3,5-dibromobenzyl)amino)cyclohexanol hydrochloride(cas: 23828-92-4) is a medication indicated to alleviate chest congestion associated with conditions that include bronchitis, pneumonia, bronchospasm asthma, cough, and allergy.COA of Formula: C13H19Br2ClN2O Preclinically, ambroxol, the active ingredient of Mucosolvan, has been shown to increase respiratory tract secretion.
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