Allen, Luke B.; Genaro-Mattos, Thiago C.; Anderson, Allison; Porter, Ned A.; Mirnics, Karoly; Korade, Zeljka published the artcile< Amiodarone Alters Cholesterol Biosynthesis through Tissue-Dependent Inhibition of Emopamil Binding Protein and Dehydrocholesterol Reductase 24>, COA of Formula: C27H44O, the main research area is amiodarone cholesterol toxicity; 8-dehydrocholesterol; cholesterol; desmosterol; emopamil binding protein; zymostenol; zymosterol.
Amiodarone is prescribed for the treatment and prevention of irregular heartbeats. Although effective in clin. practice, the long-term use of amiodarone has many unwanted side effects, including cardiac, pulmonary, hepatic, and neurol. toxicities. Our objective was to elucidate effects of amiodarone exposure on the cholesterol metabolism in cultured neuronal and non-neuronal cells and in individuals taking amiodarone. We observed that amiodarone increases distinct cholesterol precursors in different cell types in a dose-dependent manner. In liver and kidney cell lines, amiodarone causes increase in desmosterol levels, and in primary cortical neurons and astrocytes, amiodarone increases zymosterol, zymostenol, and 8-dehydrocholesterol (8-DHC). We conclude that amiodarone inhibits two enzymes in the pathway, emopamil binding protein (EBP) and dehydrocholesterol reductase 24 (DHCR24). Cortical neurons and astrocytes are more sensitive to amiodarone than liver and kidney cell lines. We confirmed the inhibition of EBP enzyme by analyzing the sterol intermediates in EBP-deficient Neuro2a cells vs. amiodarone-treated control Neuro2a cells. To determine if the cell culture experiments have clin. relevance, we analyzed serum samples from amiodarone users. We found that in patient serum samples containing detectable amount of amiodarone there are elevated levels of the sterol precursors zymosterol, 8-DHC, and desmosterol. This study illustrates the need for close monitoring of blood biochem. during prolonged amiodarone use to minimize the risk of side effects.
ACS Chemical Neuroscience published new progress about Astrocyte. 434-16-2 belongs to class alcohols-buliding-blocks, and the molecular formula is C27H44O, COA of Formula: C27H44O.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts