Xu, Siliang; Luo, Huaiyong; Chen, Haiwen; Guo, Jianbin; Yu, Bolun; Zhang, Huan; Li, Weitao; Chen, Weigang; Zhou, Xiaojin; Huang, Li; Liu, Nian; Lei, Yong; Liao, Boshou; Jiang, Huifang published the artcile< Optimization of extraction of total trans-resveratrol from peanut seeds and its determination by HPLC>, HPLC of Formula: 501-36-0 , the main research area is tran resveratrol peanut extraction optimization; high performance liquid chromatography; peanut seed; response surface methodology; trans-resveratrol.
Resveratrol, a stilbene phytoalexin in plants, is believed to benefit human health. In this study, an optimized enzyme-assisted method was developed to extract the total content of trans-resveratrol (free or combined with glucose) in peanut seeds, followed by detection using high-performance liquid chromatog. The extraction process was optimized by Box-Behnken design and response surface methodol. The optimized enzyme concentration, digestion time, pH, and temperature were 3.02 g/L, 57.06 min, 5.88, and 51.05°C, resp. Validation tests indicated that the exptl. yield of trans-resveratrol was 0.183 ± 0.007μg/g with a relative standard deviation of 3.87% (n = 5) under the optimal condition, which was closely agreed with the predicted value (0.182μg/g). The recoveries obtained from the spiked samples were varied from 89.4 to 103.9%. Therefore, this study will provide a useful method for quantification of total trans-resveratrol in peanut seeds.
Journal of Separation Science published new progress about Arachis hypogaea. 501-36-0 belongs to class alcohols-buliding-blocks, and the molecular formula is C14H12O3, HPLC of Formula: 501-36-0 .
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts