COA of Formula: C10H14O2On October 31, 2007 ,《Purification and characterization of alkylcatechol 2,3-dioxygenase from butylphenol degradation pathway of Pseudomonas putida MT4》 appeared in Journal of Bioscience and Bioengineering. The author of the article were Takeo, Masahiro; Nishimura, Munehiro; Takahashi, Hana; Kitamura, Chitoshi; Kato, Dai-ichiro; Negoro, Seiji. The article conveys some information:
Alkylcatechol 2,3-dioxygenase was purified from the cell extract of recombinant Escherichia coli JM109 harboring the alkylcatechol 2,3-dioxygenase gene (bupB) cloned from the butylphenol-degrading bacterium Pseudomonas putida MT4. The purified enzyme (BupB) showed relative meta-cleavage activities for the following catechols: catechol (100%), 4-methylcatechol (572%), 4-n-butylcatechol (185%), 4-n-hexylcatechol (53%), 4-n-heptylcatechol (45%), 4-n-nonylcatechol (10%), 4-tert-butylcatechol (0%), and 3-methylcatechol (33%). The kinetic parameters, namely, Km and Vmax, for catechol, 4-methylcatechol, and 4-n-butylcatechol, were 23.4, 8.4, and 6.5 μM and 25.8, 76.9, and 18.0 U mg-1, resp. These results suggest that BupB has broad substrate specificity for 4-n-alkylcatechols.4-Butylbenzene-1,2-diol(cas: 2525-05-5COA of Formula: C10H14O2) was used in this study.
4-Butylbenzene-1,2-diol(cas: 2525-05-5) belongs to organoboron compounds. Organoboron compounds are versatile intermediates and as such are some of the most important classes of reagents in modern organic chemistry.COA of Formula: C10H14O2 Organoboron compounds are less toxic than organostannane reagents, and unlike alkynylzinc and magnesium, many organoboron compounds possess remarkable oxidative and thermal stabilities.
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