Michel, Olga; Blasiak, Piotr; Saczko, Jolanta; Kulbacka, Julita; Drag-Zalesinska, Malgorzata; Rzechonek, Adam published the artcile< Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture>, Electric Literature of 1492-18-8, the main research area is chondrosarcoma metastasis primary cell culture electropermeabilization; chondrosarcoma; electroporation; lung metastasis; primary cell cultures.
Primary cell cultures are challenging, but reliable model reflecting tumor response in vitro. The study was designed to examine if the increased electropermeabilization can overcame initial drug insensitivity in chondrosarcoma cells from lung metastasis. We established a primary cell culture and evaluated the cytotoxic impact of four drugs-cisplatin (CDDP), camptothecin, 2-methoxyestradiol, and leucovorin calcium (LeuCa). After determination of parameters allowing for electropermeabilization, we performed electrochemotherapy in vitro with the least toxic drugs-CDDP and LeuCa. Although combining CDDP and leucovorin together increased their toxicity and supported apoptosis, application of pulsed elec. fields (PEFs) brought no advantage for their efficacy. The study emphasizes the need for introduction of primary cell cultures into studies on pulse elec. fields as model frequently less sensitive to PEF-based treatments than continuous cell lines.
Biotechnology and Applied Biochemistry published new progress about Animal tissue culture, primary. 1492-18-8 belongs to class alcohols-buliding-blocks, and the molecular formula is C20H21CaN7O7, Electric Literature of 1492-18-8.
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