Jing, Tingting; Zhang, Na; Gao, Ting; Zhao, Mingyue; Jin, Jieyang; Chen, Yongxian; Xu, Miaojing; Wan, Xiaochun; Schwab, Wilfried; Song, Chuankui published the artcile< Glucosylation of (Z)-3-hexenol informs intraspecies interactions in plants: A case study in Camellia sinensis>, HPLC of Formula: 78-70-6, the main research area is Camellia UGT Z hexenol pest control; airborne (Z)-3-hexenol; glucosyltransferase; overexpression; site-directed mutagenesis; tea plant.
Plants emit a variety of volatiles in response to herbivore attack, and (Z)-3-hexenol and its glycosides have been shown to function as defense compounds Although the ability to incorporate and convert (Z)-3-hexenol to glycosides is widely conserved in plants, the enzymes responsible for the glycosylation of (Z)-3-hexenol remained unknown until today. In this study, uridine-diphosphate-dependent glycosyltransferase (UGT) candidate genes were selected by correlation anal. and their response to airborne (Z)-3-hexenol, which has been shown to be taken up by the tea plant. The allelic proteins UGT85A53-1 and UGT85A53-2 showed the highest activity towards (Z)-3-hexenol and are distinct from UGT85A53-3, which displayed a similar catalytic efficiency for (Z)-3-hexenol and nerol. A single amino acid exchange E59D enhanced the activity towards (Z)-3-hexenol, whereas a L445M mutation reduced the catalytic activity towards all substrates tested. Transient overexpression of CsUGT85A53-1 in tobacco significantly increased the level of (Z)-3-hexenyl glucoside. The functional characterization of CsUGT85A53 as a (Z)-3-hexenol UGT not only provides the foundation for the biotechnol. production of (Z)-3-hexenyl glucoside but also delivers insights for the development of novel insect pest control strategies in tea plant and might be generally applicable to other plants.
Plant, Cell & Environment published new progress about Camellia sinensis. 78-70-6 belongs to class alcohols-buliding-blocks, and the molecular formula is C10H18O, HPLC of Formula: 78-70-6.
Referemce:
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Alcohols – Chemistry LibreTexts