Engleder, Matthias; Mueller, Monika; Kaluzna, Iwona; Mink, Daniel; Schuermann, Martin; Leitner, Erich; Pichler, Harald; Emmerstorfer-Augustin, Anita published the artcile< Exploring Castellaniella defragrans linalool (de)hydratase-isomerase for enzymatic hydration of alkenes>, Quality Control of 78-70-6, the main research area is Castellaniella defragrans; dehydratase; geraniol; hydratase; isomerase; linalool; monoterpene hydration; myrcene; tertiary alcohol.
Acyclic monoterpenes constitute a large and highly abundant class of secondary plant metabolites and are, therefore, attractive low-cost raw materials for the chem. industry. To date, numerous biocatalysts for their transformation are known, giving access to highly sought-after monoterpenoids. In view of the high selectivity associated with many of these reactions, the demand for enzymes generating com. important target mols. is unabated. Here, linalool (de)hydratase-isomerase (Ldi, EC 4.2.1.127) from Castellaniella defragrans was examined for the regio- and stereoselective hydration of the acyclic monoterpene β-myrcene to (S)-(+)-linalool. Expression of the native enzyme in Escherichia coli allowed for identification of bottlenecks limiting enzyme activity, which were investigated by mutating selected residues implied in enzyme assembly and function. Combining these analyses with the recently published 3D structures of Ldi highlighted the precisely coordinated reduction-oxidation state of two cysteine pairs in correct oligomeric assembly and the catalytic mechanism, resp. Subcellular targeting studies upon fusion of Ldi to different signal sequences revealed the significance of periplasmic localization of the mature enzyme in the heterologous expression host. This study provides biochem. and mechanistic insight into the hydration of β-myrcene, a nonfunctionalized terpene, and emphasizes its potential for access to scarcely available but com. interesting tertiary alcs.
Molecules published new progress about 78-70-6. 78-70-6 belongs to class alcohols-buliding-blocks, and the molecular formula is C10H18O, Quality Control of 78-70-6.
Referemce:
Alcohol – Wikipedia,
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