Schulz, Patrick; Dueck, Benjamin; Mourot, Alexandre; Hatahet, Lina; Fendler, Klaus published the artcile< Measuring ion channels on solid supported membranes>, Reference of 35564-86-4, the main research area is measurement ion channel solid supported membrane.
Application of solid supported membranes (SSMs) for the functional investigation of ion channels is presented. SSM-based electrophysiol., which has been introduced previously for the investigation of active transport systems, is expanded for the anal. of ion channels. Membranes or liposomes containing ion channels are adsorbed to an SSM and a concentration gradient of a permeant ion is applied. Transient currents representing ion channel transport activity are recorded via capacitive coupling. The authors demonstrate the application of the technique to liposomes reconstituted with the peptide cation channel gramicidin, vesicles from native tissue containing the nicotinic acetylcholine receptor, and membranes from a recombinant cell line expressing the ionotropic P2X2 receptor. It is shown that stable ion gradients, both inside as well as outside directed, can be applied and currents are recorded with an excellent signal/noise ratio. For the nicotinic acetylcholine receptor and the P2X2 receptor excellent assay quality factors of Z’ = 0.55 and Z’ = 0.67, resp., are obtained. This technique opens up new possibilities in cases where conventional electrophysiol. fails like the functional characterization of ion channels from intracellular compartments. It also allows for robust fully automatic assays for drug screening.
Biophysical Journal published new progress about Adsorption. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Reference of 35564-86-4.
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