Luo, Yitao; Liu, Zhengyuan; Zeng, Yujie; Zhang, Yuxiao; Luan, Yujing; Ma, Li; Chen, Li; Zou, Lin; Yang, Jingmin; Huang, Zhibin; Rao, Yulan; Zhang, Chengqiang published the artcile< A reliable tool for detecting 7-dehydrocholesterol and cholesterol in human plasma and its use in diagnosis of Smith-Lemli-Opitz syndrome>, Product Details of C27H44O, the main research area is Smith-Lemli-Opitz syndrome; antioxidants; biomarkers; inborn errors of metabolism; microwave-assisted derivatization.
Background : Smith-Lemli-Opitz syndrome is a birth defect caused by the deficiency of 7-dehydrocholesterol reductase in cholesterol biosynthesis pathway, which leads to accumulation of 7-dehydrocholesterol and reduction of cholesterol in body fluids. To effectively diagnose Smith-Lemli-Opitz syndrome and monitor therapy, a reliable method for simultaneous detection of 7-dehydrocholesterol and cholesterol is needed. Methods : In the presence of antioxidants (2,6-ditert-butyl-4-methylphenol and triphenylphosphine), 50 μL of human plasma were hydrolyzed at 70°C for 40 min with 1 M potassium hydroxide in 90% ethanol, and then 7-dehydrocholesterol and cholesterol were extracted by 600 μL of n-hexane for three times. After microwave-assisted derivatization with 70 μL of N,O-bis(trimethylsilyl)trifluoroacetamide at 460 W for 3 min, the analytes were measured by gas chromatog.-mass spectrometry. Results : The limits of detection were 100 ng/mL for 7-dehydrocholesterol and 300 ng/mL for cholesterol. Good linearity was obtained in the range of 1-600 μg/mL for 7-dehydrocholesterol and 10-600 μg/mL for cholesterol, which completely covered the biochem. levels of Smith-Lemli-Opitz syndrome patients that have been reported. Conclusion : A time-saving and accurate gas chromatog. with mass spectrometry based method was developed for the determination of 7-dehydrocholesterol and cholesterol in human plasma, which also serves as a useful tool for Smith-Lemli-Opitz syndrome diagnosis, treatment, and research.
Journal of Separation Science published new progress about 434-16-2. 434-16-2 belongs to class alcohols-buliding-blocks, and the molecular formula is C27H44O, Product Details of C27H44O.
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