Gas-Pascual, Elisabet; Ichikawa, Hiroshi Travis; Sheikh, Mohammed Osman; Serji, Mariam Isabella; Deng, Bowen; Mandalasi, Msano; Bandini, Giulia; Samuelson, John; Wells, Lance; West, Christopher M. published the artcile< CRISPR/Cas9 and glycomics tools for toxoplasma glycobiology>, Safety of (2S,3S,4R,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is genome editing CRISPR Cas9 glycome Toxoplasma human infection; CRISPR/Cas; Toxoplasma gondii; glycan; glycobiology; glycogene; glycomics; glycosyltransferase; mass spectrometry (MS); parasitology; protein glycosylation.
Infection with the protozoan parasite Toxoplasma gondii is a major health risk owing to birth defects, its chronic nature, ability to reactivate to cause blindness and encephalitis, and high prevalence in human populations. Unlike most eukaryotes, Toxoplasma propagates in intracellular parasitophorous vacuoles, but like nearly all other eukaryotes, Toxoplasma glycosylates many cellular proteins and lipids and assembles polysaccharides. Toxoplasma glycans resemble those of other eukaryotes, but species-specific variations have prohibited deeper investigations into their roles in parasite biol. and virulence. The Toxoplasma genome encodes a suite of likely glycogenes expected to assemble N-glycans, O-glycans, a C-glycan, GPI-anchors, and polysaccharides, along with their precursors and membrane transporters. To investigate the roles of specific glycans in Toxoplasma, here we coupled genetic and glycomics approaches to map the connections between 67 glycogenes, their enzyme products, the glycans to which they contribute, and cellular functions. We applied a double-CRISPR/Cas9 strategy, in which two guide RNAs promote replacement of a candidate gene with a resistance gene; adapted MS-based glycomics workflows to test for effects on glycan formation; and infected fibroblast monolayers to assess cellular effects. By editing 17 glycogenes, we discovered novel Glc0-2-Man6-GlcNAc2-type N-glycans, a novel HexNAc-GalNAc-mucin-type O-glycan, and Tn-antigen; identified the glycosyltransferases for assembling novel nuclear O-Fuc-type and cell surface Glc-Fuc-type O-glycans; and showed that they are important for in vitro growth. The guide sequences, editing constructs, and mutant strains are freely available to researchers to investigate the roles of glycans in their favorite biol. processes.
Journal of Biological Chemistry published new progress about Amplicon Role: BUU (Biological Use, Unclassified), BIOL (Biological Study), USES (Uses) (DHFR). 3458-28-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C6H12O6, Safety of (2S,3S,4R,5R)-2,3,4,5,6-Pentahydroxyhexanal.
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