An efficient 2-keto-L-gulonic acid whole-cell biotransformation system built on the characterization of L-sorbose dehydrogenase was written by Li, Fan;Wang, Cai-Yun;Zhang, Meng-Yue;Zhang, Yi-Xuan. And the article was included in Molecular Catalysis in 2022.Recommanded Product: 149-32-6 This article mentions the following:
There are five L-sorbose dehydrogenases (SDH_2764, SDH_2744, SDH_0718, SDH_1927, SDH_0337) in Ketogulonicigenium vulgare SPU B805 and three L-sorbose dehydrogenases (SDH_02251, SDH_02271, SDH_P100164) in K. robustum SPU_B003, which play essential roles in the classical two-step fermentation for 2-keto-L-gulonic acid (2-KGA, the precursor of vitamin C) synthesis. In this work, the above eight SDH enzymes were cloned and purified, and their optimal pH values and reaction temperatures, kinetic properties (Km and Kcat), thermal stabilities, substrate spectra, and effects of metal ions were determined Eight SDH-harboring recombinant E.coli strains were verified to be capable to catalyze L-sorbose to 2-KGA. Addnl., the mol. docking results showed that the product 2-KGA could inhibit SDH activity by competing for the same hydrogen bonding sites with substrate L-sorbose. Finally, a novel and efficient whole-cell recycling catalysis system to overcome the product inhibition was proposed, the yield of 2-KGA reached up to 80.12 g/L with the productivity of 6.68 g/L/h. The results provide a promising prospect for selection and modification sorbose dehydrogenase for construction a one-step biotransformation system of 2-KGA. In the experiment, the researchers used many compounds, for example, (2R,3S)-rel-Butane-1,2,3,4-tetraol (cas: 149-32-6Recommanded Product: 149-32-6).
(2R,3S)-rel-Butane-1,2,3,4-tetraol (cas: 149-32-6) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Recommanded Product: 149-32-6
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