Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology was written by Mills, Carolyn E.;Waltmann, Curt;Archer, Andre G.;Kennedy, Nolan W.;Abrahamson, Charlotte H.;Jackson, Alexander D.;Roth, Eric W.;Shirman, Sasha;Jewett, Michael C.;Mangan, Niall M.;Olvera de la Cruz, Monica;Tullman-Ercek, Danielle. And the article was included in Nature Communications in 2022.Safety of 1,2-Propanediol This article mentions the following:
Abstract: Engineering subcellular organization in microbes shows great promise in addressing bottlenecks in metabolic engineering efforts; however, rules guiding selection of an organization strategy or platform are lacking. Here, we study compartment morphol. as a factor in mediating encapsulated pathway performance. Using the 1,2-propanediol utilization microcompartment (Pdu MCP) system from Salmonella enterica serovar Typhimurium LT2, we find that we can shift the morphol. of this protein nanoreactor from polyhedral to tubular by removing vertex protein PduN. Anal. of the metabolic function between these Pdu microtubes (MTs) shows that they provide a diffusional barrier capable of shielding the cytosol from a toxic pathway intermediate, similar to native MCPs. However, kinetic modeling suggests that the different surface area to volume ratios of MCP and MT structures alters encapsulated pathway performance. Finally, we report a microscopy-based assay that permits rapid assessment of Pdu MT formation to enable future engineering efforts on these structures. In the experiment, the researchers used many compounds, for example, 1,2-Propanediol (cas: 57-55-6Safety of 1,2-Propanediol).
1,2-Propanediol (cas: 57-55-6) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Safety of 1,2-Propanediol
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