Low molecular weight PEI-based polycationic gene vectors via Michael addition polymerization with improved serum-tolerance was written by Xun, Miao-Miao;Xiao, Ya-Ping;Zhang, Ji;Liu, Yan-Hong;Peng, Qi;Guo, Qian;Wu, Wan-Xia;Xu, Yong;Yu, Xiao-Qi. And the article was included in Polymer in 2015.Safety of Diethyleneglycoldiacrylate This article mentions the following:
A series of polycationic gene delivery vectors were synthesized via Michael addition from low mol. weight PEI and linking compounds with various heteroatom compositions Agarose gel electrophoresis results reveal that these polymers can well condense plasmid DNA and can protect DNA from degradation by nuclease. The formed polyplexes, which are stable toward serum, have uniform spherical nanoparticles with appropriate sizes around 200-350 nm and zeta-potentials about +40 mV. In vitro experiments show that these polymers have lower cytotoxicity and higher transfection efficiency than 25 kDa PEI. Furthermore, the title materials exhibit excellent serum tolerance. With the present of 10% serum, up to 19 times higher transfection efficiency than PEI was obtained, and no obvious decrease of TE was observed even the serum concentration was raised to >40%. Flow cytometry and confocal microscopy studies also demonstrate the good serum tolerance of the materials. In the experiment, the researchers used many compounds, for example, Diethyleneglycoldiacrylate (cas: 4074-88-8Safety of Diethyleneglycoldiacrylate).
Diethyleneglycoldiacrylate (cas: 4074-88-8) belongs to alcohols. The oxygen atom of the strongly polarized O―H bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Safety of Diethyleneglycoldiacrylate
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts