Novel substrates for efficient enzymatic transglycosylation by Bacillus circulans was written by Komba, Shiro;Ito, Yukishige. And the article was included in Canadian Journal of Chemistry in 2002.SDS of cas: 60463-12-9 This article mentions the following:
To develop transglycosylation for efficient preparation of N-acetyllactosamine (Galβ(1→4)GlcNAc, LacNAc), β-galactosidase mediated transglycosylation using novel substrates Bacillus Circulans was explored. To make transglycosylation entropically favorable over hydrolysis, donor (lactose or galactose) and acceptor (N-acetylglucosamine, GlcNAc) components were connected to a single mol. For that purpose, 1,2- and 1,3-benzenedimethanol and 2-hydroxy-5-nitro- and 5-hydroxy-2-nitro-benzyl alc. were screened as linkers and enzymic transglycosylation was examined under several conditions. In the case of 2-hydroxy-5-nitro-benzyl connected substrate 40, an indication of the occurrence of intramol. transglycosylation was observed, and the desired product (58) was obtained in 26% isolated yield. The same reaction in the presence of CMP sialic acid and α-(2→6)-sialyltransferase gave sialyl LacNAc 87 in one pot in 39% isolated yield. Addnl., the effect of the C-2 substituent of the acceptor component was briefly examined using substrates containing NHAlloc (72), NHTroc (73), and N3 (74) groups. Although the occurrence of intramol. transglycosylation was not clear in these cases, disaccharides 81-83 were obtained in reasonable yields. In the experiment, the researchers used many compounds, for example, 3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9SDS of cas: 60463-12-9).
3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Under carefully controlled conditions, simple alcohols can undergo intermolecular dehydration to give ethers. This reaction is effective only with methanol, ethanol, and other simple primary alcohols.SDS of cas: 60463-12-9
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts