β-Galactosidase-catalyzed intramolecular transglycosylation was written by Komba, Shiro;Ito, Yukishige. And the article was included in Tetrahedron Letters in 2001.Computed Properties of C7H7NO4 This article mentions the following:
A novel transglycosylation strategy for efficient preparation of N-acetyllactosamine (Galβ1→4GlcNAc, LacNAc) and sialyl LacNAc was developed using β-galactosidase from Bacillus circulans. In order to minimize the competing hydrolysis by forcing the enzymic transglycosylation to proceed in an intramol. manner, a novel substrate carrying the donor (galactose) and the acceptor (N-acetylglucosamine) components linked via a 2-hydroxy-5-nitro-benzylalc. derived tether was prepared Treatment with β-galactosidase from B. circulans afforded the transglycosylation product in 26% yield. Furthermore, addition of sialyltransferase and CMP-sialic acid to this system gave sialyl LacNAc in 39% yield. In the experiment, the researchers used many compounds, for example, 3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9Computed Properties of C7H7NO4).
3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Computed Properties of C7H7NO4
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts