Zhang, Xiang-mei published the artcileIdentification of vitamin C precursor 2-keto-L-gulonic acid, Formula: C6H10O7, the publication is Zhongguo Weisheng Jianyan Zazhi (2014), 24(10), 1399-1400, database is CAplus.
The objective of this paper was to establish the method system for identification of 2-keto-L-gulonic acid (2-KLG) in fermentation broth, and to distinguish 2-KLG from its byproducts (5-DKG and 2-DKG). The standard substance and fermentation product samples were analyzed by thin layer chromatog.(TLC), HPLC and m.p. determination, then the results were compared to confirm the consistency of the three methods in identification of 2-KLG. The TLC results indicated that the Rf value, color and size of chromatogram were concordant between fermentation product and the standard substance of 2-KLG. HPLC results showed that the fermentation product exhibited concordant retention time (8 min), m.p.(170 °C or so) and pH value with standard substance of 2-KLG, but different from 2-DKG and 5-DKG. This method system could identify the existence of 2-KLG in unknown fermentation products, with great significance in screening of new strains of 2-KLG from further fermentation of D-glucose.
Zhongguo Weisheng Jianyan Zazhi published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C5H7NO, Formula: C6H10O7.
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