Hou, Wei published the artcileCloning, expression and activity analysis of sndh-sdh gene cluster of Gluconobacter oxydans, Category: alcohols-buliding-blocks, the publication is Shengwu Jishu Tongxun (2012), 23(3), 389-392, database is CAplus.
Objective: To clone the sndh-sdh gene cluster from Gluconobacter oxydans H763 and investigate the expression and biol. activity of sorbosone dehydrogenase-sorbose dehydrogenase (SNDH-SDH) in Escherichia coli DH5α and G. oxydans 621H. Methods & Results: The sndh-sdh gene cluster, including promoter, structural gene and terminator sequence, was directly amplified using PCR method from G. oxydans H763 genome DNA. Then the 3533 bp amplification product was retrieved and ligated into the vector pMD18T and transformed into E. coli DH5α. Its expression and biol. activity were examined by DCIP assay when sorbose or xylose was used as substrate. After the cell lysis was added to the DCIP detection solution, its color changed from bluish green to yellow, which suggested that the expression product had dehydrogenase activity. The vector pBBR1MCS2-sndh-sdh was constructed and conjugated into G. oxydans 621H. After the recombinant strain was cultivated in the medium containing sorbitol or sorbose as substrate, the metabolic products in the supernatant were assayed by thin-layer chromatog., and chromatoplate showed the 2-keto-L-gulonic acid (2-KLG) spots. Conclusion: The above results indicated that the SNDH-SDH was expressed in E. coli DH5α and G. oxydans 621H.
Shengwu Jishu Tongxun published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Category: alcohols-buliding-blocks.
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