Zhu, Quan-Fei published the artcileIn-Depth Annotation Strategy of Saturated Hydroxy Fatty Acids Based on Their Chromatographic Retention Behaviors and MS Fragmentation Patterns, Application of 17-Hydroxyheptadecanoic acid, the main research area is hydroxy fatty acid annotation LC MS.
Hydroxy fatty acids are a class of bioactive compounds in a variety of organisms. The identification of hydroxy fatty acids in biol. samples has still been a challenge because of their low abundance, high structural similarity, and limited availability of authentic hydroxy fatty acid standards Here, we present a strategy for the annotation of saturated monohydroxyl fatty acids (OH-FAs) based on the integration of chromatog. retention rules and MS2 fragmentation patterns. Thirty-nine authentic OH-FA standards were used to investigate their retention behavior on a reversed-phase stationary phase (C18) of liquid chromatog., and we found that their retention simultaneously follows two kinds of “”carbon number rules””. Using the “”carbon number rules””, the retention index (RI) of all OH-FAs that contain carbon numbers from 8 to 18 (C8-18) can be predicted. Addnl., by studying the MS2 fragmentation of OH-FAs under collision-induced dissociation, we found that the intensity ratio (IR) of the characteristic fragment ions ([M + H]+-63 and [M + H]+-45) is closely related to the position of the hydroxyl group on the OH-FA structure, which is helpful to further identify and confirm the OH-FA isomers. As a result, 97 of 107 potential OH-FAs detected in honey, human serum, and rice seedling by chem. isotope labeling-assisted liquid chromatog.-mass spectrometry were annotated upon the RI matching and IR confirming. Furthermore, in order to simplify the annotation process of OH-FAs, we constructed an OH-FA library to facilitate the annotation of OH-FAs. Overall, this study provides a new and promising tool for the in-depth annotation of OH-FA isomers.
Analytical Chemistry (Washington, DC, United States) published new progress about Blood analysis. 13099-34-8 belongs to class alcohols-buliding-blocks, name is 17-Hydroxyheptadecanoic acid, and the molecular formula is C17H34O3, Application of 17-Hydroxyheptadecanoic acid.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts