Biochemical Journal published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.
Ogier, Sally Ann published the artcileNovel ligands for the affinity labeling of luteinizing hormone releasing hormone receptors, Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biochemical Journal (1989), 258(3), 881-8, database is CAplus and MEDLINE.
A number of novel LH-RH analogs incorporating biotin together with potential covalent attachment sites were synthesized. Those based on the des-Gly10-[D-Lys6]-LH-RH ethylamide peptide backbone resulted in the most useful characteristics of binding to the LH-RH receptor in rat anterior pituitary gland membranes. Of these, des-Gly10-[biotinyl-aminoethylglycyl-D-Lys6]-LHRH ethylamide (XBAL) gave the best specific:nonspecific binding ratio, with 44% of total binding being specific with a Kd of 131 pM as determined by Scatchard anal. Two methods were used to covalently crosslink these analogs with the LH-RH receptor; photoaffinity labeling and the use of homobifunctional N-hydroxysuccinimide ester crosslinkers. The photoaffinity analogs gave poor specific:nonspecific binding ratios. Of the chem. crosslinkers tested, ethylene glycolbis(succinimidylsuccinate) (EGS) was the most efficient at covalently linking the 125I-XBAL bound to the LH-RH receptor site. At an EGS concentration of 5 mM, 23% of the specific binding of 125I-XBAL was covalently crosslinked.
Biochemical Journal published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts