Process Biochemistry (Oxford, United Kingdom) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C38H74Cl2N2O4, COA of Formula: C6H10O7.
Zhu, Yibo published the artcileA high throughput method to screen companion bacterium for 2-keto-L-gulonic acid biosynthesis by co-culturing Ketogulonicigenium vulgare, COA of Formula: C6H10O7, the publication is Process Biochemistry (Oxford, United Kingdom) (2012), 47(9), 1428-1432, database is CAplus.
Bacillus megaterium is widely used as companion bacterium in the two-step biosynthesis of 2-keto-L-gulonic acid (2-KLG) by Ketogulonicigenium vulgare. To screen efficiently target companion strains from large numbers of random mutants, a screen method based on spectrophotometry and 24-well microtiter plates was developed and validated on an integrated library of 450 transposon random insertional mutants and two sporulation-defective mutants. The co-culture processes were classified into three groups (low, intermediate and high performance) by K-mean clustering anal. In addition, mutant m71 was successfully screened out from the library. The substrate conversion ratio of m71 and K. vulgare co-culture process after 72 h was decreased by about 38% compared with that of the wild-type co-culture process in 750 mL flasks. These results indicated that the proposed high throughput method is feasible for screening target companions for the co-culture process of 2-KLG biosynthesis.
Process Biochemistry (Oxford, United Kingdom) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C38H74Cl2N2O4, COA of Formula: C6H10O7.
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