Liang, YaoYao published the artcileDirected evolution of PobR allosteric transcription factor to generate biosensor for 4-hydroxymandelic acid, Formula: C8H8O3, the publication is World Journal of Microbiology & Biotechnology (2022), 38(6), 104, database is CAplus and MEDLINE.
Hydroxy-mandelic acid (HMA) is widely applied in pharmaceuticals, food and cosmetics. In this study, we aimed to develop an allosteric transcription factors (aTFs) based biosensor for HMA. PobR, an aTF for HMA analog 4-hydroxybenzoic acid, was used to alter its selectivity and create novel aTFs responsive to HMA by directed evolution. We established a PobR mutant library with a capacity of 550,000 mutants using error-prone PCR and Megawhop PCR. Through our screening, two mutants were obtained with responsiveness to HMA. Anal. of each missense mutation indicating residues 122-126 were involved in its PobR ligand specificity. These results showed the effectiveness of directed evolution in switching the ligand specificity of a biosensor and improving HMA production
World Journal of Microbiology & Biotechnology published new progress about 90-64-2. 90-64-2 belongs to alcohols-buliding-blocks, auxiliary class Carboxylic acid,Benzene,Alcohol,Natural product, name is 2-Hydroxy-2-phenylacetic acid, and the molecular formula is C8H8O3, Formula: C8H8O3.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
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