Antharavally, Babu S. published the artcileEfficient removal of detergents from proteins and peptides in a spin column format, Synthetic Route of 85618-21-9, the publication is Analytical Biochemistry (2011), 416(1), 39-44, database is CAplus and MEDLINE.
Detergents are commonly used in protein-chem. protocols and may be necessary for protein extraction, solubilization, and denaturation; however, their presence interferes with many downstream anal. techniques, including mass spectrometry (MS). To enable downstream anal., it is critical to remove unbound detergents from protein and peptide samples. In this study, the authors describe a high-performance resin that offers exceptional detergent removal for proteins and peptides. When used in a spin column format, this resin dramatically improves protein and peptide MS results by more than 95% removal of 1-5% detergents, including SDS, sodium deoxycholate, Chaps, Triton X-100, Triton X-114, NP-40, Brij-35, octyl glucoside, octyl thioglucoside, and lauryl maltoside, with high recovery of proteins and peptides. Postcolumn liquid chromatog.-tandem MS (LC-MS/MS) anal. of trypsin digests of bovine serum albumin (BSA) and HeLa cell lysate revealed excellent sequence coverage, indicating successful removal of detergent from the peptides. Matrix-assisted laser desorption/ionization (MALDI)-MS anal. of unprocessed and processed samples further confirmed efficient removal of detergents. The advantages of this method include speed (<15 min), efficient detergent removal, and high recovery of proteins and peptides.
Analytical Biochemistry published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Synthetic Route of 85618-21-9.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts