Grabosch, Carsten published the artcileGlycoarrays by a New Tandem Noncovalent-Covalent Modification of Polystyrene Microtiter Plates and their Interrogation with Live Cells, Recommanded Product: (2R,3S,4S,5S,6R)-2-(Hydroxymethyl)-6-(4-isothiocyanatophenoxy)tetrahydro-2H-pyran-3,4,5-triol, the publication is ChemBioChem (2012), 13(13), 1874-1879, S1874/1-S1874/18, database is CAplus and MEDLINE.
Thiourea bridging was used for fabrication of glycoarrays on polystyrene microtiter plates, to allow assaying of carbohydrate recognition in a optimally easy and flexible way. For the noncovalent modification of polystyrene microtiter plates, dodecylamine and dodecyl isothiocyanate were selected. This first immobilization step was followed by thiourea bridging with a suitable carbohydrate derivative and subsequent carbohydrate-specific read out. For the testing system, mannose-specific bacterial adhesion was employed with green fluorescent protein (GFP)-tagged Escherichia coli bacteria (pPKLl 162). A selection of inhibitors of type 1 fimbriae-mediated bacterial adhesion was used. Tandem noncovalent-covalent modification of polystyrene microtiter plates led to stable glycoarrays that yield valuable and valid data in adhesion inhibition tests with live bacterial cells. Nonspecific adhesion to polystyrene is excluded in this protocol. In addition to polystyrene plates, polypropylene (PP) microtiter plates were tested by the same approach and showed results that were in good accordance with the assays on analogously modified polystyrene plates.
ChemBioChem published new progress about 96345-79-8. 96345-79-8 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Gal and Man, name is (2R,3S,4S,5S,6R)-2-(Hydroxymethyl)-6-(4-isothiocyanatophenoxy)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C13H15NO6S, Recommanded Product: (2R,3S,4S,5S,6R)-2-(Hydroxymethyl)-6-(4-isothiocyanatophenoxy)tetrahydro-2H-pyran-3,4,5-triol.
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