Cho, Hea-Young published the artcileSimultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography, HPLC of Formula: 328-90-5, the publication is Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (2003), 798(2), 257-264, database is CAplus and MEDLINE.
A rapid, selective and sensitive HPLC method was developed and validated for the simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl HOBz (HTB), in rat and human plasma. HPLC anal. was carried out using a 5-μm particle size, C18-bonded SiO2 column and MeCN-MeOH-H2O (25:10:65, volume/volume/v) as the mobile phase and UV detection at 234 nm. Furosemide was used as the internal standard The method involved extraction with an MeCN-CHCl3 mixture (60:40, volume/volume) and evaporation to dryness with N stream. The chromatograms showed good resolution and sensitivity and no interferences by plasma constituents. The mean absolute recovery for human plasma was 93.5 ± 4.2% for triflusal and 98.5 ± 3.1% for HTB. The lower limits of quantification of triflusal and HTB in human plasma were 20 and 100 ng/mL, resp. The calibration curves in human plasma were linear over the concentration range 0.02-5.0 μg/mL for triflusal and 0.1-200.0 μg/mL for HTB with correlation coefficients >0.999 and with inter- or intra-day coefficients of variation (CV) not exceeding 10.0%. This assay procedure was applied to the study of metabolite pharmacokinetics of triflusal and HTB in rat and human.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about 328-90-5. 328-90-5 belongs to alcohols-buliding-blocks, auxiliary class Trifluoromethyl,Fluoride,Carboxylic acid,Benzene,Phenol, name is 2-Hydroxy-4-(trifluoromethyl)benzoic acid, and the molecular formula is C8H5F3O3, HPLC of Formula: 328-90-5.
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