Yang, Weichao published the artcileA plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-L-gulonic acid production, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Brazilian Journal of Microbiology (2017), 48(3), 397-402, database is CAplus and MEDLINE.
A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-L-gulonic acid (2-KLG). The screening methodol. took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining pos. mutants. Theproposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.
Brazilian Journal of Microbiology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C8H5F3N4, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.
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