Murphy, Ann published the artcileChemically stabilized trypsin used in dipeptide synthesis, Application In Synthesis of 70539-42-3, the publication is Biotechnology and Bioengineering (1998), 58(4), 364-373, database is CAplus and MEDLINE.
Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester), such that approx. 8 out of 14 lysines per trypsin mol. were modified. This derivative (EG trypsin) was more stable than native between 30° and 70°: T50 values were 59° and 46°, resp. EG trypsin’s half-life of 25 min at 55° was fivefold greater than native’s. EG trypsin had a decreased rate of autolysis and retained more activity in aqueous mixtures of 1,4-dioxan, DMF, dimethylsulfoxide, and acetonitrile. EG trypsin had lower Km values for both amide and ester substrates; its kcat values for two amides (PhCO-Arg-NHC6H4NO2-4 and Cbz-Gly-Gly-Arg-NMC; NMC = 7-amino-4-methylcoumarin) increased, whereas its kcat value for an ester (Cbz-Lys-SCOPh) decreased slightly. The specific activity (kcat/Km) of EG trypsin was increased for both amide and ester substrates. EG trypsin gave higher yields and reaction rates than the native trypsin in kinetically controlled synthesis of PhCO-Arg-Leu-NH2 in acetonitrile and in t-butanol. Highest peptide yields occurred with EG trypsin in 95% acetonitrile, where 90% of the substrate was converted to product. No peptide synthesis occurred in 95% DMF with either form of trypsin.
Biotechnology and Bioengineering published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Application In Synthesis of 70539-42-3.
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