Padmanabhan, Aparna published the artcileStructural characterization of exopolysaccharide from Streptococcus thermophilus ASCC 1275, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is Streptococcus exopolysaccharide purification dialysis; exopolysaccharide; glycosyl linkage; nuclear magnetic resonance (NMR); structure.
In this study, we purified and characterized exopolysaccharide (EPS) produced by a high-EPS-producing dairy starter bacterium, Streptococcus thermophilus ASCC 1275. Crude EPS was extracted from S. thermophilus ASCC 1275 and partially purified using dialysis. Further purification and fractionation of exopolysaccharide was conducted using HPLC on a Superose 6 column (Cytiva/Global Life Sciences Solutions, Marlborough, MA). Glycosyl composition anal., linkage anal. along with 1-dimensional and 2-dimensional NMR spectroscopy were performed to deduce the structure of EPS. Three fractions (F) obtained from gel permeation chromatog. were termed F1 (2.6%), F2 (45.8%), and F3 (51.6%) with average mol. weights of approx. 511, 40, and 5 kDa, resp. Monosaccharide composition anal. revealed the dominance of glucose, galactose, and mannose in all 3 fractions. Major linkages observed in F3 were terminal galactopyranosyl (t-Gal), 3-linked glucopyranosyl (3-Glc), 3-linked galactofuranosyl (3-Galf), and 3,6-linked glucopyranosyl (3,6-Glc) and major linkages present in F2 were 4-Glc (48 mol%), followed by terminal mannopyranosyl (t-Man), 2- + 3-linked mannopyranosyl (2-Man+3-Man), and 2,6-linked mannopyranosyl (2,6-Man; total ∼28 mol%). The 1-dimensional and 2-dimensional NMR spectroscopy revealed that F2 comprised mannans linked by (1→2) linkages and F3 consisted of linear chains of α-D-glucopyranosyl (α-D-Glcp), β-D-glucopyranosyl (β-D-Glcp), and β-D-galactofuranosyl (β-D-Galf) connected by (1→3) linkages; branching was through (1→6) linkage in F3. A possible structure of EPS in F2 and F3 was proposed.
Journal of Dairy Science published new progress about Dialysis. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.
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