Hadjikinova, Raina published the artcileDevelopment and validation of HPLC-RID method for determination of sugars and polyols, SDS of cas: 64519-82-0, the main research area is dessert food sugar polyol HPLC RID analysis.
The aim of the current study was to develop and validate a rapid, sensitive and accurate method for simultaneous determination of sugars (glucose, fructose, sucrose and lactose) and polyols (erythritol, mannitol, sorbitol, isomalt and xylitol) in low-calorie and no-sugar added desserts by high pressure liquid chromatog. coupled with refractive index detector (RID). The best chromatog. separation was performed on Pb2+ Shodex Sugars SP0810 operating at 80°C and mobile phase distilled water with flow rate 0.5 mL/min. Validation procedure includes for linearity, precision and accuracy of the method. The developed method offers excellent linearity in wide concentration range (0.1-5 mg/mL) for all tested carbohydrates with R2>0.997. Limit of detection (LOD) and limit of quantification (LOQ) for nine analytes were in the range of 0.01-0.17 mg/mL and 0.03-0.56 mg/mL, resp. HPLC-RID method showed very good repeatability (RSD >5 %) and reproducibility. The developed method was successfully applied for quantification of sugars and sugar alcs. in dessert foods. The results obtained from the analyzed real samples showed the possibility of the proposed method to be used for routine anal. of sugars and sugar alcs. in low-calorie, no-sugar added dessert foods and pharmaceutical formula only with one injection of the sample.
Journal of Pharmaceutical Sciences and Research published new progress about Desserts. 64519-82-0 belongs to class alcohols-buliding-blocks, name is (3R,4R,5R)-6-(((2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)hexane-1,2,3,4,5-pentaol, and the molecular formula is C12H24O11, SDS of cas: 64519-82-0.
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