Tahka, Sari published the artcileImmobilization of proteolytic enzymes on replica-molded thiol-ene micropillar reactors via thiol-gold interaction, Synthetic Route of 7575-23-7, the main research area is chymotrypsin immobilization micropillar reactor; Enzyme immobilization; Gold nanoparticles; Mass spectrometry; Microfluidics; Microreactors; Thiol-enes.
We introduce rapid replica molding of ordered, high-aspect-ratio, thiol-ene micropillar arrays for implementation of microfluidic immobilized enzyme reactors (IMERs). By exploiting the abundance of free surface thiols of off-stoichiometric thiol-ene compositions, we were able to functionalize the native thiol-ene micropillars with gold nanoparticles (GNPs) and these with proteolytic α-chymotrypsin (CHT) via thiol-gold interaction. The micropillar arrays were replicated via PDMS soft lithog., which facilitated thiol-ene curing without the photoinitiators, and thus straightforward bonding and good control over the surface chem. (number of free surface thiols). The specificity of thiol-gold interaction was demonstrated over allyl-rich thiol-ene surfaces and the robustness of the CHT-IMERs at different flow rates and reaction temperatures using bradykinin hydrolysis as the model reaction. The product conversion rate was shown to increase as a function of decreasing flow rate (increasing residence time) and upon heating of the IMER to physiol. temperature Owing to the effective enzyme immobilization onto the micropillar array by GNPs, no further purification of the reaction solution was required prior to mass spectrometric detection of the bradykinin hydrolysis products and no clogging problems, commonly associated with conventional capillary packings, were observed The activity of the IMER remained stable for at least 1.5 h (continuous use), suggesting that the developed protocol may provide a robust, new approach to implementation of IMER technol. for proteomics research. [Figure not available: see fulltext.].
Analytical and Bioanalytical Chemistry published new progress about Enzymic hydrolysis. 7575-23-7 belongs to class alcohols-buliding-blocks, name is Pentaerythritol tetra(3-mercaptopropionate), and the molecular formula is C17H28O8S4, Synthetic Route of 7575-23-7.
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