Liu, Youxia published the artcileComprehensive analysis of aberrantly expressed profiles of mRNA and its relationship with serum galactose-deficient IgA1 level in IgA nephropathy, COA of Formula: C6H12O6, the main research area is gene expression galactose IgA1 IgA nephropathy; Differential gene expression; Galactose-deficient IgA1; IgA nephropathy; RNA-deep sequencing; mRNA microarray.
IgA nephropathy (IgAN) is the leading cause of end-stage kidney disease. Previous mRNA microarray profiling studies of IgAN revealed inconsistent data. We sought to identify the aberrantly expressed genes and biol. pathways by integrating IgAN gene expression datasets in blood cells and performing systematically exptl. validation. We also explored the relationship between target genes and galactose-deficient IgA1 (Gd-IgA1) in IgAN. We retrieved Gene Expression Omnibus (GEO) datasets of IgAN. Gene Ontol. (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used for functional anal. Deep sequencing on RNA isolated from B cells was used for microarray validation. The relationship between target mRNA expressions and Gd-IgA1 levels in serum were also studied. Three studies with microarray expression profiling datasets met our inclusion criteria. We identified 655 dyregulated genes, including 319 up-regulated and 336 down-regulated genes in three GEO datasets with a total of 35 patients of IgAN and 19 healthy controls. Based on biol. process in GO term, these dyregulated genes are mainly related to pentose-phosphate shunt, non-oxidative branch, post-embryonic camera-type eye development and leukocyte activation. KEGG pathway anal. of microarray data revealed that these aberrantly expressed genes were enriched in human T-cell leukemia virus 1 infection, proteoglycans in cancer, intestinal immune network for IgA production and autophagy. We further performed deep sequencing on mRNAs isolated from B cells of an independent set of five patients with IgAN and three healthy persons with the same clin. and demog. characteristics. Seventy-seven genes overlapped with 655 differentially regulated genes mentioned above, including 43 up-regulated and thirty-four down-regulated genes. We next investigated whether these genes expression correlated with Gd-IgA1 levels in IgAN patients. Pearson correlation analyses showed PTEN (phosphatase and tensin homolog) was the most powerful gene neg. correlated with Gd-IgA1 levels. These results demonstrated that dyregulated genes in patients with IgAN were enriched in intestinal immune network for IgA production and autophagy process, and PTEN in B cells might be involved in the mechanism of Gd-IgA1 production
Journal of Translational Medicine published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (DEF8). 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, COA of Formula: C6H12O6.
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