Foong, Choon Pin published the artcileEngineered mutants of a marine photosynthetic purple nonsulfur bacterium with increased volumetric productivity of polyhydroxyalkanoate bioplastics, Related Products of alcohols-buliding-blocks, the main research area is mutagenesis polyhydroxyalkanoate bioplastic Rhodovulum; Rhodovulum sulfidophilum; biopolymer; fluorescence-activated cell sorting; genome-wide mutagenesis; microbial cell factory; strain improvement.
Polyhydroxyalkanoates (PHAs) are green and sustainable bioplastics that could replace petrochem. synthetic plastics without posing environmental threats to living organisms. In addition, sustainable PHA production could be achieved using marine photosynthetic purple nonsulfur bacteria (PNSBs) that utilize natural seawater, sunlight, carbon dioxide gas, and nitrogen gas for growth. However, PHA production using marine photosynthetic PNSBs has not been economically feasible yet due to its high cost and low productivity. In this work, strain improvement, using genome-wide mutagenesis coupled with high-throughput screening via fluorescence-actived cell sorting, we were able to create Rhodovulum sulfidophilium mutants with enhanced volumetric PHA productivity, with an up to 1.7-fold increase. The best selected mutants (E6 and E6M4) reached the stationary growth phase 1 day faster and accumulated the maximum PHA content 2 days faster than the wild type. Maximizing volumetric PHA productivity before the stationary growth phase is indeed an addnl. advantage for R. sulfidophilum as a growth-associated PHA producer.
ACS Synthetic Biology published new progress about Fluorescence-activated cell sorting. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Related Products of alcohols-buliding-blocks.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts