Black, Alyson P. published the artcileA Novel Mass Spectrometry Platform for Multiplexed N-Glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-Glycan Imaging, SDS of cas: 59-23-4, the main research area is mass spectrometry imaging glycoprotein biomarker antibody panel.
A new platform for N-glycoprotein anal. from serum that combines matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) workflows with antibody slide arrays is described. Antibody panel based (APB) N-glycan imaging allows for the specific capture of N-glycoproteins by antibodies on glass slides and N-glycan anal. in a protein-specific and multiplexed manner. Development of this technique has focused on characterizing two abundant and well-studied human serum glycoproteins, alpha-1-antitrypsin and IgG. Using purified standard solutions and 1 μL samples of human serum, both glycoproteins can be immunocaptured and followed by enzymic release of N-glycans. N-Glycans are detected with a MALDI FT-ICR mass spectrometer in a concentration-dependent manner while maintaining specificity of capture. Importantly, the N-glycans detected via slide-based antibody capture were identical to that of direct anal. of the spotted standards As a proof of concept, this workflow was applied to patient serum samples from individuals with liver cirrhosis to accurately detect a characteristic increase in an IgG N-glycan. This novel approach to protein-specific N-glycan anal. from an antibody panel can be further expanded to include any glycoprotein for which a validated antibody exists. Addnl., this platform can be adapted for anal. of any biofluid or biol. sample that can be analyzed by antibody arrays.
Analytical Chemistry (Washington, DC, United States) published new progress about Blood analysis. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts