Lacroix, Pauline M. published the artcileHPLC and NMR methods for the quantitation of the (R)-enantiomer in (-)-(S)-timolol maleate drug raw materials, Recommanded Product: 4-Morpholino-1,2,5-thiadiazol-3-ol, the publication is Chirality (1994), 6(6), 484-91, database is CAplus.
HPLC and 1N-NMR methods for the quantitation of the (R)-enantiomer in (-)-(S)-timolol maleate were developed and validated. The HPLC method requires a 25 cm × 4.6 nm 5 μm Chiracel OD-H (cellulose tris-3,5-dimethylphenylcarbamate) column, a mobile phase of 0.2% (volume/volume) diethylamine and 4% (volume/volume) isopropanol in hexane at a flow rate of 1 mL/min and UV detection at 297 nm. A system suitability test was devised to verify the separation of the (R)- and (S)-enantiomers of timolol from other drug-related impurities. The NMR method requires the use of a high-field NMR spectrometer (> 360 MHz) and a chiral solvating agent, (-)-(R)-2,2,2-trifluoro-1-(9-anthrylethanol)(R-TFAE). The limits of quantitation were 0.5% and 0.2% (m/m) for HPLC and NMR, resp. The methods were applied to the determination of the (R)-enantiomer in eight lots of raw material. The results for the two methods were in very good agreement, with results ranging from 0.1 to 4.1% (m/m) by HPLC and none detected to 4.3% (m/m) by NMR. The USP method for sp. rotation was found to be unsuitable for detecting the presence of low levels of the (R)-enantiomer in (-)-(S)-timolol maleate.
Chirality published new progress about 30165-97-0. 30165-97-0 belongs to alcohols-buliding-blocks, auxiliary class Morpholine,Thiadiazole,Alcohol, name is 4-Morpholino-1,2,5-thiadiazol-3-ol, and the molecular formula is C6H9N3O2S, Recommanded Product: 4-Morpholino-1,2,5-thiadiazol-3-ol.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts