Dhabaria, Avantika published the artcileA High-Efficiency Cellular Extraction System for Biological Proteomics, Computed Properties of 85618-21-9, the publication is Journal of Proteome Research (2015), 14(8), 3403-3408, database is CAplus and MEDLINE.
Recent developments in quant. high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of the biochem. analyses of cellular reactions, protein-protein interactions, and small-mol.-drug discovery. These approaches depend on cellular proteome extraction that preserves native protein activities. Here, we systematically analyzed mech. methods of cell lysis and phys. protein extraction to identify those that maximize the extraction of cellular proteins while minimizing their denaturation. Cells were mech. disrupted using Potter-Elvehjem homogenization, probe- or adaptive-focused acoustic sonication, and were in the presence of various detergents, including polyoxyethylene ethers and esters, glycosides, and zwitterions. Using fluorescence spectroscopy, biochem. assays, and mass spectrometry proteomics, we identified the combination of adaptive focused acoustic (AFA) sonication in the presence of a binary poloxamer-based mixture of octyl-β-glucoside and Pluronic F-127 to maximize the depth and yield of the proteome extraction while maintaining native protein activity. This binary poloxamer extraction system allowed for native proteome extraction comparable in coverage to the proteomes extracted using denaturing SDS or guanidine-containing buffers, including the efficient extraction of all major cellular organelles. This high-efficiency cellular extraction system should prove useful for a variety of cell biochem. studies, including structural and functional proteomics.
Journal of Proteome Research published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Computed Properties of 85618-21-9.
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