Schulze, Bastian published the artcileColumn bleed in the analysis of highly polar substances: an overlooked aspect in HRMS, HPLC of Formula: 622-40-2, the publication is Analytical and Bioanalytical Chemistry (2020), 412(20), 4837-4847, database is CAplus and MEDLINE.
Abstract: To close the “anal. gap” in the liquid chromatog. (LC) anal. of highly polar substances, two techniques which have been suggested earlier were tested in terms of retention factors and detection limits: hydrophilic interaction liquid chromatog. (HILIC) and mixed-mode chromatog. (MMC). A substance mix of 55 analytes ranging from logD – 8.2 to 3.4 and 17 different LC columns, also comprising addnl. reversed-phase columns were used. Contrary to most reversed-phase columns, column bleed has been identified as an important factor, which may cause serious restrictions during high-resolution mass spectrometric detection (HRMS). We found that highly abundant background masses continuously eluting from the columns heavily influence ion transmission to the detector. As a result, the linear dynamic range as well as the sensitivity decreases and thus limits the HRMS applicability of some columns. We therefore recommend a thorough investigation of ion transmission during HRMS method development. This will help to maintain the high potential of HRMS in terms of qual. and quant. screening anal.
Analytical and Bioanalytical Chemistry published new progress about 622-40-2. 622-40-2 belongs to alcohols-buliding-blocks, auxiliary class Morpholine,Alcohol, name is 2-Morpholinoethanol, and the molecular formula is C23H20BN, HPLC of Formula: 622-40-2.
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