Optogenetic reprogramming of carbon metabolism using light-powering microbial proton pump systems was written by Toya, Yoshihiro;Hirono-Hara, Yoko;Hirayama, Hidenobu;Kamata, Kentaro;Tanaka, Ryo;Sano, Mikoto;Kitamura, Sayaka;Otsuka, Kensuke;Abe-Yoshizumi, Rei;Tsunoda, Satoshi P.;Kikukawa, Hiroshi;Kandori, Hideki;Shimizu, Hiroshi;Matsuda, Fumio;Ishii, Jun;Hara, Kiyotaka Y.. And the article was included in Metabolic Engineering in 2022.Computed Properties of C9H18O5S The following contents are mentioned in the article:
In microbial fermentative production, ATP regeneration, while crucial for cellular processes, conflicts with efficient target chem. production because ATP regeneration exhausts essential carbon sources also required for target chem. biosynthesis. To wrestle with this dilemma, we harnessed the power of microbial rhodopsins with light-driven proton pumping activity to supplement with ATP, thereby facilitating the bioprodn. of various chems. We first demonstrated a photo-driven ATP supply and redistribution of metabolic carbon flows to target chem. synthesis by installing already-known delta rhodopsin (dR) in Escherichia coli. In addition, we identified novel rhodopsins with higher proton pumping activities than dR, and created an engineered cell for in vivo self-supply of the rhodopsin-activator, all-trans-retinal. Our concept exploiting the light-powering ATP supplier offers a potential increase in carbon use efficiency for microbial productions through metabolic reprogramming. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Computed Properties of C9H18O5S).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Tertiary alcohols cannot be oxidized at all without breaking carbon-carbon bonds, whereas primary alcohols can be oxidized to aldehydes or further oxidized to carboxylic acids.Computed Properties of C9H18O5S
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts