Immobilization of microbial consortium for glutaric acid production from lysine was written by Gao, Cong;Wang, Jiaping;Guo, Liang;Hu, Guipeng;Liu, Jia;Song, Wei;Liu, Liming;Chen, Xiulai. And the article was included in ChemCatChem in 2021.Synthetic Route of C9H18O5S The following contents are mentioned in the article:
Glutaric acid is an important C5 platform compound for the synthesis of nylon polymers. However, its production titer and cost are limited by the need for expensive cofactors addition as well as the difficulty of reusing microbial catalysts. Herein, to address these problems, two regeneration systems each for NAD(P)+ and α-ketoglutaric acid were introduced into a five-enzyme cadaverine pathway for a whole-cell biocatalytic approach. We demonstrated the efficient conversion of lysine to glutaric acid in a synthetic microbial consortium manner, producing 73.2 g/L glutaric acid with a 74% molar yield in a 5-L bioreactor scale. Moreover, with a colloidal chitin-based immobilization, the engineered microbial consortium exhibited good stability and reusability in a five-cycle bioconversion. This study provides an efficient microbial platform for glutaric acid production by developing a cheaper and universal cell immobilization approach. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Synthetic Route of C9H18O5S).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. The oxygen atom of the strongly polarized O―H bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.Synthetic Route of C9H18O5S
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts