Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B6 in energy drinks and dietary supplements was written by Zeng, Lu;Xu, Xinxin;Song, Shanshan;Xu, Liguang;Liu, Liqiang;Xiao, Jing;Xu, Chuanlai;Kuang, Hua. And the article was included in Nano Research in 2022.Synthetic Route of C8H10ClNO3 The following contents are mentioned in the article:
We designed and synthesized novel haptens to produce monoclonal antibodies (mAb) against vitamin B6 (VB6). A group-specific mAb (2F9) that recognized pyridoxine (PN), pyridoxamine (PM), and pyridoxal (PL) was prepared using a homologous strategy with 50% maximal inhibitory concentration (IC50) values of 106.60, 250.57, and 400.11 ng/mL, resp. Based on this, a gold nanoparticles (AuNPs)-based immunochromatog. strip (ICS) test was established for the detection of VB6 in energy drinks and B-vitamin complex tablets. The developed ICS test results could be semi-quant. evaluated by the naked eye within 10 min, and displayed the visual limit of detection (vLOD) values of 250, 500, and 1,000 ng/mL for PN, PM, and PL, resp. For quant. anal., the results obtained by strip reader, with calculated LOD values for PN, PM, and PL were 14.10, 55.58, and 56.25 ng/mL, resp. Com. energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid chromatog. Overall, the developed AuNPs-based immunochromatog. sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Synthetic Route of C8H10ClNO3).
3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Synthetic Route of C8H10ClNO3
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts