Stauber, Gregory B. published the artcileCharacterization and affinity cross-linking of receptors for human recombinant lymphotoxin (tumor necrosis factor-β) on a human histiocytic lymphoma cell line, U-937, COA of Formula: C18H20N2O12, the publication is Journal of Biological Chemistry (1989), 264(6), 3573-6, database is CAplus and MEDLINE.
Recombinant human lymphotoxin (rhLT) was purified and used to examine its receptors on the human histiocytic lymphoma cell line U-937. The rhLT was radioiodinated by the IODO GEN method to a specific activity of 60 μCi/μg. The specific binding reached a plateau within 10, 60, and 180 min at 37, 23, and 4°, resp. Scatchard anal. revealed the presence of a single class of high affinity receptors with an apparent Kd of 0.6 nm and a capacity of 33,000 binding sites/cell. The binding of 125I-labeled rhLT to U-937 cells was inhibited by excess unlabeled rhLT or recombinant human tumor necrosis factor-α(rhTNF), suggesting a common receptor for both mols. The crosslinking of the receptor to rhLT revealed 2 distinct bands at approx. mol. masses of 100,000 and 120,000 daltons. Affinity crosslinking of U-937 cells with 125I-labeled rhTNF, however, provided only a single band with a mol. mass of about 100,000 daltons. Thus, the manner in which rhLT interacts with its receptor is perhaps somewhat different from that of rhTNF.
Journal of Biological Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C16H25BO2, COA of Formula: C18H20N2O12.
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