Metabolic engineering of Escherichia coli with electron channelling for the production of natural products was written by Park, Seon Young;Eun, Hyunmin;Lee, Mun Hee;Lee, Sang Yup. And the article was included in Nature Catalysis in 2022.HPLC of Formula: 367-93-1 The following contents are mentioned in the article:
The biosynthesis of natural products often requires eukaryotic cytochrome P450s (P450s) in combination with P 450 reductase, in phys. proximity, to perform electron-transfer reactions. Unfortunately, functional expression of eukaryotic P450s in bacteria remains generally difficult. Here we report an electron channeling strategy based on the application of Photorhabdus luminescens CipB scaffold protein, which allows efficient electron transfer between P450s and reductases by bringing these enzymes in close proximity. The general applicability of this electron channeling strategy is proved by developing recombinant Escherichia coli strains producing lutein, (+)-nootkatone, apigenin and L-3,4-dihydroxyphenylalanine (L-DOPA), each of which requires P450s in its biosynthetic pathway. The production titers are then further enhanced by increasing the haem pathway flux or by optimization of the culture conditions. Remarkably, the final lutein strain produced 218.0 mg l-1 of lutein with a productivity of 5.01 mg l-1 h-1 in fed-batch fermentation under optimized culture conditions. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1HPLC of Formula: 367-93-1).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Under carefully controlled conditions, simple alcohols can undergo intermolecular dehydration to give ethers. This reaction is effective only with methanol, ethanol, and other simple primary alcohols.HPLC of Formula: 367-93-1
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts