Genome-wide protein-DNA interaction site mapping in bacteria using a double-stranded DNA-specific cytosine deaminase was written by Gallagher, Larry A.;Velazquez, Elena;Peterson, S. Brook;Charity, James C.;Radey, Matthew C.;Gebhardt, Michael J.;Hsu, FoSheng;Shull, Lauren M.;Cutler, Kevin J.;Macareno, Keven;de Moraes, Marcos H.;Penewit, Kelsi M.;Kim, Jennifer;Andrade, Pia A.;LaFramboise, Thomas;Salipante, Stephen J.;Reniere, Michelle L.;de Lorenzo, Victor;Wiggins, Paul A.;Dove, Simon L.;Mougous, Joseph D.. And the article was included in Nature Microbiology in 2022.HPLC of Formula: 367-93-1 The following contents are mentioned in the article:
DNA-protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these interactions on a genome scale in bacteria are laborious and capture only a snapshot of binding events. We devised a facile method for mapping DNA-protein interaction sites in vivo using the double-stranded DNA-specific cytosine deaminase toxin DddA. In 3D-seq (DddA-sequencing), strains containing DddA fused to a DNA-binding protein of interest accumulate characteristic mutations in DNA sequence adjacent to sites occupied by the DNA-bound fusion protein. High-depth sequencing enables detection of sites of increased mutation frequency in these strains, yielding genome-wide maps of DNA-protein interaction sites. We validated 3D-seq for four transcription regulators in two bacterial species, Pseudomonas aeruginosa and Escherichia coli. We show that 3D-seq offers ease of implementation, the ability to record binding event signatures over time and the capacity for single-cell resolution This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1HPLC of Formula: 367-93-1).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.HPLC of Formula: 367-93-1
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts