Diaporthe/Phomopsis longicolla degrades an array of bisphenol analogues with secreted laccase was written by Baluyot, Jobriell C.;Santos, Hanna Keith;Batoctoy, Dessa Camille R.;Torreno, Vicenzo Paolo M.;Ghimire, Leela B.;Joson, Santiago Emil A. IV;Obusan, Marie Christine M.;Yu, Eizadora T.;Bela-ong, Dennis B.;Gerona, Roy R.;Velarde, Michael C.. And the article was included in Microbiological Research in 2022.Quality Control of 4,4′-Methylenediphenol The following contents are mentioned in the article:
With recent initiatives to ban bisphenol A (BPA) in certain com. products, manufacturers shifted to the production and use of BPA analogs. However, some of these BPA alternatives still possess endocrine disruptive activities. Many fungal enzymes are known to biodegrade phenolic compounds, such as BPA. However, the activity of these enzymes on BPA analogs remains unexplored. This study reports a secreted laccase from the endophytic fungus Diaporthe longicolla capable of degrading an impressive range of bisphenol analogs. The secreted crude enzymes are optimally active at pH 5 from 39°C to 60°C, efficiently degrading BPA as well as BPA analogs BPB, BPC, BPE and BPF. A purified form of laccase was identified from the crude fungal extract using FPLC and peptide sequencing. Furthermore, BPA induced the expression of this D. longicolla laccase gene. Overall, this paper demonstrated that the crude laccase enzyme from D. longicolla metabolizes BPA and select analogs, implicating the potential role of this fungus to remove environmental bisphenols. This study involved multiple reactions and reactants, such as 4,4′-Methylenediphenol (cas: 620-92-8Quality Control of 4,4′-Methylenediphenol).
4,4′-Methylenediphenol (cas: 620-92-8) belongs to alcohols. The oxygen atom of the strongly polarized O―H bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. Tertiary alcohols cannot be oxidized at all without breaking carbon-carbon bonds, whereas primary alcohols can be oxidized to aldehydes or further oxidized to carboxylic acids.Quality Control of 4,4′-Methylenediphenol
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