Chemical denaturants induced folding unfolding pathway of the recombinant zebrafish dihydrofolate reductase was written by Acharya, Vaishali V.;Verma, Anita Kamra;Chaudhuri, Pratima. And the article was included in Indian Journal of Biochemistry & Biophysics in 2022.Related Products of 367-93-1 The following contents are mentioned in the article:
Denaturation of proteins plays a crucial part in cellular activities. In this study, we have investigated the folding unfolding pathways of zebrafish dihydrofolate reductase (zDHFR) in presence of different chem. denaturants which were found to be an influential factor for the refolding yield by UV-visible spectrophotometric anal. The activity change of zDHFR has been observed in presence of three different denaturants like Acetic Acid (AcOH), Sodium Dodecyl Sulfate (SDS), and Ethanol (C2H5OH). Spectrophotometric anal. reveals that protein unfolded completely at different concentrations and times by these denaturants. The spontaneous refolding experiments of chem. denatured zDHFR were also conducted to verify the spontaneous refolding yield. These investigations have helped us to decipher a picture about the denaturants contributing to achieving the refolding yield. We observed that acetic acid is a stronger denaturant among all, and the spontaneous refolding yields were higher from SDS denaturation. In the light of the above findings, higher spontaneous refolding yields were obtained from the low concentration of denaturants. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Related Products of 367-93-1).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Related Products of 367-93-1
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