Study on the interaction between pyridoxal and CopC by multi-spectroscopy and docking methods was written by Song, Zhen;Liu, Jin;Hou, Yuxin;Yuan, Wen;Yang, Binsheng. And the article was included in Spectrochimica Acta in 2019.COA of Formula: C8H10ClNO3 The following contents are mentioned in the article:
The interaction between pyridoxal hydrochloride (HQ) and apoCopC was investigated using Fourier transform IR spectroscopy (FTIR), isothermal titration calorimetry (ITC), CD (CD), fluorescence spectroscopy, three-dimensional (3D) fluorescence spectroscopy, fluorescence lifetime, TNS fluorescence and docking methods. FTIR, CD, TNS fluorescence and fluorescence lifetime experiments suggested that the apoCopC conformation was altered by HQ with an increase in the random coil content and a reduction in the β-sheet content. In addition, the data from fluorescence spectroscopy, 3D fluorescence spectroscopy and mol. docking revealed that the binding site of HQ was located in the hydrophobic area of apoCopC, and a red shift of the HQ fluorescence spectra was observed Furthermore, ITC and fluorescence quenching data manifested that the binding ratio of HQ and apoCopC was 1:1, and the forming constant was calculated to be (7.06 ± 0.21) × 105 M-1. The thermodn. parameters ΔH and ΔS suggested that the formation of a CopC-HQ complex depended on the hydrophobic force. Furthermore, the average binding distance between tryptophan in apoCopC and HQ was determined by means of Forster non-radioactive resonance energy transfer and mol. docking. The results agreed well with each other. As a redox switch in the modulation of copper, the interaction of apoCopC with small mols. will affect the action of the redox switch. These findings could provide useful information to illustrate the copper regulation mechanism. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5COA of Formula: C8H10ClNO3).
3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.COA of Formula: C8H10ClNO3
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts