New pyridoxal based chemosensor for selective detection of zinc ion: Application in live cell imaging and phosphatase activity response was written by Mandal, Senjuti;Sikdar, Yeasin;Maiti, Dilip K.;Sanyal, Ria;Das, Debasis;Mukherjee, Abhishek;Mandal, Sushil Kumar;Biswas, Jayanta Kumar;Bauza, Antonio;Frontera, Antonio;Goswami, Sanchita. And the article was included in Journal of Photochemistry and Photobiology, A: Chemistry in 2017.Reference of 65-22-5 The following contents are mentioned in the article:
Although a variety of fluorescence based chemosensors have been utilized for selective detection of Zn2+, pyridoxal containing simple Schiff bases still remained less explored. Here, we combine pyridoxal hydrochloride and 1,2-diaminocyclohexane to generate a new sensor mol., H4PydChda (5-Hydroxymethyl-4-((2-(5-hydroxymethyl-2-methylpyridin-3-hydroxy-4-ylethylene)-amino)-cyclohexylimino)-methyl-2-methylpyridin-3-ol). Chemosensor H4PydChda exhibits selective turn-on type response in presence of Zn2+ in ethanol-water mixture at physiol. pH. Appreciable fluorescence enhancement occurs upon addition of Zn2+ to H4PydChda as a result of inhibited C=N isomerisation and excited state intramol. proton transfer leading to efficient chelation enhanced fluorescence. The relevant properties, including reversibility, life time measurements and detection limit have been determined for the sensor system. The exptl. and theor. supports in terms of 1H and 13C NMR spectroscopy and DFT/ TDDFT study are provided to establish the binding mode of H4PydChda to Zn2+. H4PydChda was employed as a sensor for detection of Zn2+ in Human gastric adenocarcinoma AGS cells. Moreover, the resulting probe-Zn2+complex shows convincing phosphatase activity kcat = 21.59 s-1 opening a promising avenue for further research. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Reference of 65-22-5).
3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Reference of 65-22-5
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts