Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry was written by Lee, Ju Yong;Wang, Hua;Pyrgiotakis, Georgios;DeLoid, Glen M.;Zhang, Zhenyuan;Beltran-Huarac, Juan;Demokritou, Philip;Zhong, Wenwan. And the article was included in Analytical and Bioanalytical Chemistry in 2018.Recommanded Product: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate The following contents are mentioned in the article:
Nanoparticles (NPs) tend to adsorb matrix mols. like proteins and lipids incubated with biol. fluids, forming a biol. corona. While the formation and functions of protein corona have been studied extensively, little attention has been paid to lipid adsorption on NPs. However, lipids are also abundantly present in biol. fluids and play important roles in processes like cell signaling and angiogenesis. Therefore, in this study, we established the anal. procedure for study of lipid adsorption on three different types of NPs in two matrixes: human serum and heavy cream, using nanoflow liquid chromatog.-mass spectrometry (nanoflow LC-MS). Serum was chosen to represent the common environment the NPs would be present once entering human body, and heavy cream was the representative food matrix NPs may be added to improve the color or taste. Steps of liquid-liquid extraction were established and optimized to achieve maximum recovery of the adsorbed, standard lipids from the NPs. Then, the LC-MS/MS method was developed to attain base-line separation of the standard lipids that represent the major lipid classes. At last, the lipid adsorption profiles of the three NPs were compared. We found that the lipid adsorption profile on the same type of NP was significantly different between the two matrixes. The established method will help us investigate lipid adsorption on addnl. NPs and reveal how it could be affected by the physiochem. properties of NPs and the presence of proteins and other components in the biol. matrix. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Recommanded Product: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate).
(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Recommanded Product: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate
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